Translated as an Ile (ATT)>Val (GTT) substitution in the ARV7 variant protein. Sequencing of ARVamplicons revealed a single nucleotide substitution within CE3 in lung and placental tissue samples that could be In addition, four novel ARVs containing a cryptic exon 9 (CE9) were detected in MDA-MB-453 and VCaP cells. Four ARVs (V1, V3, V7, V9) were detected to some degree in almost all cell lines and tissues. Herein, the expression of five previously identified ARV transcripts with documented transcriptional capacity (AR-V1, -V3, -V4, -V7, and -V9) was examined in 6 breast (MFM223, MDA-MB-453, MDA-MB-231, ZR75.1, MCF-7, T47D), two prostate (VCaP, LNCaP), and one liver (HepG2) cancer cell lines, a human embryonic kidney cell line (HEK293), and a panel of RNAs representing 21 different human tissues. We hypothesised that ARVs are also expressed in breast cancers and other hormone sensitive tissues.
Expression of AR splice variants (ARVs) and their role in prostate carcinogenesis has been elucidated in recent studies. AR activity inhibits breast growth and has pleiotropic actions in breast cancer that are subtype-dependent. In particular, the AR plays a critical role in the biology and pathology of the prostate gland. Received: 10 December 2013 / Accepted: 10 February 2014 # Springer Science+Business Media New York 2014Ībstract The androgen receptor (AR) is widely expressed in human tissues and has biological function in many male and female organs. Hickey & Connie Irvine & Dhilushi Dodampege Wijayakumara & Lu Lu & Wayne D. Identification of Androgen Receptor Splice Variant Transcripts in Breast Cancer Cell Lines and Human Tissues Dong Gui Hu & Theresa E.
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